Composite

Part:BBa_K4092108:Design

Designed by: Damian Alonso Espinoza Ruiz & Shani Betsymar Leyva Camacho   Group: iGEM21_Tec-Monterrey   (2021-10-20)


Toehold Bois Noir 2.1 (from team EPFL 2019) expression casette with amilCP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 96
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This cassette expression is regulated by a T7 promoter and expresses AmilCP chromoprotein when the toehold interacts with its corresponding trigger sequence



Source

Toehold RNA complementary to PlsC gene (Candidatus phytoplasma solani), T7 promoter from bacteriophage T7 and AmilCP from the coral Acropora millepora.

References